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Unchained Labs expands its focus on lipid nanoparticles with the acquisition of Blacktrace Holdings

Unchained Labs expands its focus on lipid nanoparticles with the acquisition of Blacktrace Holdings

With the acquisition of Blacktrace Holdings, Unchained Labs now has three brands in its product portfolio, including Lipid nanoparticle (LNP) formulations.

Pleasanton, United States: On Monday, July 17th, 2023, Unchained Labs, a life sciences business dedicated to providing the best tools for biologics and gene therapy for researchers, acquired Blacktrace Holdings. Blacktrace's Dolomite microfluidics have been assisting scientists with complex operations for decades. Recently, their Particle Works solutions have changed the game in terms of how researchers test lipid nanoparticle (LNP)- all while using the same methodologies and microfluidic chip.

LNPs are ideal vaccines, but they also show promise in gene therapy nut to be successful, LNPs are required to be of correct size and composition to enter cells and deliver their payload. Finding the optimum formulation for the ideal LNP needs a lot of trial and error. Researchers determine the best lipid combination, the best lipid-to-payload ratio, and the various flow rates required to mix it all together – which is a time-consuming operation. Thus, for time efficiency Particle Works LNP solutions of Blacktrace Holdings are being utilized. ALiS (Automated Library Synthesis System) is the sole option for high-throughput LNP formulation screening available. ALiS generates 96 unique, 200 µl LNP formulations in a single six-hour experiment, which may then be checked for size and encapsulation before being forwarded to payload expression tests. Once the ideal formulation is identified, ANP (Automated Nanoparticle System) takes over to fine-tune the ALiS technique, which can be scaled up to over 50 liters in 24 hours using the same reusable chip. Unchained Labs is also presently developing a GMP manufacturing solution, which it intends to complete and make accessible as soon as possible.

According to the Chairman & CEO of Blacktrace Holdings, "We have spent the past two years developing and providing a better way to make LNPs," said Mark Gilligan, CEO at Blacktrace Holdings. "Unchained Labs is committed to providing LNP researchers with tailor-made solutions and I can't think of a better home for our Particle Works solutions."

According to the Founder and CEO at Unchained Labs, "Next-generation modalities like LNPs are changing the world," said Tim Harkness, Founder and CEO at Unchained Labs. "All we think about every day is how to make the jobs of researchers developing these complex therapies easier, and we can't wait to get ALiS and ANP into the LNP labs that need them."

According to TechSci Research, the versatility of liquid nanoparticles is continually expanding as researchers are exploring new materials, fabrication methods, and applications. Unchained Labs has been established to provide LNP researchers with specialized assistance. With the Acquisition of Blacktrace Holdings, the company is expanding its product portfolio by utilizing the Automated Library Synthesis System (ALiS) and Automated Nanoparticle (ANP) System which are the two prominent system offered by the particle works brand of Blacktrace holdings.

The Automated Library Synthesis System (ALiS), a brand-new, revolutionary automated platform offered by Blacktrace holdings for high-throughput screening of LNP formulations containing genetic material, including mRNA, for early-stage development of genetic drugs and vaccines. By doing up to 96 different experiments in less than 6 hours, ALiS will dramatically speed up early-stage research and development/discovery initiatives.

Another system of Blacktrace holdings which is a 3-in-1 Automated Nanoparticle (ANP) System for protocol creation and LNP synthesis process optimization, can revolutionize lipid nanoparticle (LNP) synthesis. This cutting-edge microfluidic technology offers comprehensive process development options for vaccines, medications, gene therapies, RNA treatments, and other APIs (such as polymer-based nanoparticles), making it perfect for lipid nanoparticle encapsulation. Thus, access to this system can enhance the Unchained Labs efficiency to produce LNP.

Lipid nanoparticles have had a genuinely extraordinary influence on medicine, particularly with the invention of mRNA vaccines by firms like Moderna and Pfizer, which have been working on LNP for more than 20 years. The effectiveness of these vaccinations has been nothing short of miracle in the healthcare industry, but lipid nanoparticles are making a difference in areas other than COVID-19. Onpattro, for instance, is a medication that treats a rare genetic condition by encapsulating short-interfering RNA in lipid nanoparticles. This is just one example of how lipid nanoparticles are being utilized to influence gene expression, which is changing the treatment of some diseases. Lipid nanoparticles can also transport difficult-to-solubilize medications such as Iclusig and even smaller molecules such as doxycycline. Doxycycline, when synthesized into lipid nanoparticles, can be utilized to treat some forms of cancer.

LNP stability, drug retention in the particles, and controlled release are all critical for drug delivery applications, but they are difficult to achieve. Thus, designing novel lipids and enhancing LNP composition can open up new avenues for drug delivery applications by providing leveraging benefits to the manufacturers as well as the researchers. Ongoing research and development efforts are likely lead to the discovery of new materials, improved nanoparticle formulations, and enhanced functionalities, making liquid nanoparticles more versatile and effective with simultaneously increasing its demand in the upcoming years.

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